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American Society of Microbiology ABMM Sample Questions:

1. A patient develops a severe skin and soft tissue infection following a boating accident in the Gulf Coast.
Wound cultures grow a Gram-negative, halophilic bacterium that is oxidase-positive. The MOST likely organism is:

A) Pseudomonas aeruginosa
B) Shewanella putrefaciens
C) Vibrio vulnificus
D) Aeromonas hydrophila

2. A patient with AIDS develops a disseminated fungal infection. Blood cultures grow a yeast that produces pseudohyphae and terminal chlamydospores on cornmeal agar. The patient's CD4+ T-cell count is below 50 cells/µL. The MOST likely causative organism is:

A) Pneumocystis jirovecii
B) Cryptococcus neoformans
C) Aspergillus fumigatus
D) Candida albicans

3. A clinical laboratory manager is implementing a new automated blood culture system. Which aspect is LEAST likely to be part of the initial verification/validation process before clinical use?

A) Comparison of time-to-detection with the previous system
B) Assessment of the system's contamination rate over one year of routine use
C) Verification of detection of a panel of common bloodstream pathogens
D) Establishing workflow and training competency for laboratory staff

4. Which biosafety level (BSL) is required for clinical laboratory work involving the propagation (culture) of Coccidioides immitis/posadasii?

A) BSL-1
B) BSL-4
C) BSL-3
D) BSL-2

5. A clinical microbiology laboratory is performing quality control on antimicrobial susceptibility testing. The zone diameter for Escherichia coli ATCC 25922 tested against ciprofloxacin is consistently smaller than the acceptable range published by CLSI. The MOST likely cause of this discrepancy is:

A) Inoculation with a bacterial suspension that is too dense
B) All of the above
C) Use of expired antibiotic disks
D) Incubation at an incorrect temperature

Solutions: