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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Chemistry | 20–25% | - Carbohydrates, lipids, and enzymes - Electrolytes, acid-base, and toxicology |
| Topic 2: Blood Banking | 15–20% | - Transfusion practice and testing - Blood group systems and immunology - Blood products and components |
| Topic 3: Microbiology | 15–20% | - Bacteriology and mycology procedures - Virology and parasitology testing |
| Topic 4: Laboratory Operations | 5–10% | - Quality assessment and safety - Instrumentation and laboratory mathematics |
| Topic 5: Immunology | 5–10% | - Principles of immunology - Serologic procedures and immune diseases |
| Topic 6: Urinalysis and Other Body Fluids | 5–10% | - Microscopic analysis and disease correlation - Physical and chemical testing |
| Topic 7: Hematology | 20–25% | - Laboratory testing and hemostasis - Physiology and disease states |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
1. Once substance H is developed, the addition of the sugar N-acetylgalactosamine to the terminal position of the chain gives the molecule "A" antigenic activity.
Which specific terminal sugar causes a red cell to have A antigenic activity?
A) N-acetylgalactosamine
B) Galactose plus glucose
C) Glucose
D) Galactose
2. Blood plasma will contain coagulation proteins, but not in a clotted tube where serum is present.
Blood serum contains each of the following substances except:
A) Water
B) Electrolytes
C) Sugars
D) Coagulation proteins
3. This drawing depicts beta thalassemia minor B+/B. In Beta thalassemia minor B+/B, one beta gene locus is partially deleted or inactive.
Hematology
This drawing depicts which beta chain genotype ?
A) Beta thalassemia minor
B) Delta-beta thalassemia minor
C) Beta thalassemia major
D) Beta thalassemia intermedia
4. Most anticoagulants in blood collectiong tubes prevent clotting by:
Question options:
A) binding fibrinogen
B) releasing heparin
C) acting as an antithrombin agent
D) binding calcium
5. The dematiaceous molds can be broadly separated into two major groups: the agents of chromomycosis that grow more slowly, maturing only after 7 days or more of incubation, and the more rapidly growing species that most commonly are clinically insignificant commensals or contaminants when recovered from clinical specimens, but in rare situations may cause opportunistic infections called phaeohyphomycosis.
Dematiaceous molds can be broadly separated into two major groups; the agents of chromomycosis and clinically insignificant commensals or contaminants. The agents of chromomycosis grow: more slowly than; more rapidly than; or generally at the same rate as the clinically insignificant commensals or contaminants?
A) more rapidly than
B) more slowly than
C) generally at the same rate as
Solutions:
| Question # 1 Answer: A | Question # 2 Answer: D | Question # 3 Answer: A | Question # 4 Answer: D | Question # 5 Answer: B |



